Course code: KE127
Type of course: Compulsory
Level of course: Basic
Year of study: 3th year
Semester: 6th
Number of credits
allocated (student workload based): 5
Name of lecturer: Prof. Boris Pihlar, Ass. Prof.
Drago Kočar
Objective
of the course (expected learning outcomes and competences to be acquired):
Learning outcomes: Understanding of the fundamental
principles of commonly used instrumental techniques, their advantages and
limitations and will learn about possibility for their application in research
and analysis of real samples.
Competences: Students will get practical skills and experiences on the application of some important
instrumental techniques and will be able to evaluate and critically present analytical
results obtained by instrumental measurements.
Prerequisites: Basic knowledge of mathematics, physics, inorganic chemistry,
analytical chemistry, physical chemistry and instrumental methods.
Course contents:
Statistical
methods and approaches in instrumental analysis; linear and nonlinear
regression, statistical tests, analysis of variance (ANOVA), evaluation of uncertainty, accreditation and quality
assurance.
Electroanalytical
techniques for trace analysis: potentiometric sensors, coulometry, voltammetry (SDC, PP DPP, SWV), cyclic
voltammetry, stripping techniques.
Radiochemical
methods: radioactive isotopes and properties of radioactive radiation,
radiactive equilibria, activation analysis and instrumentation, application of neutron activation analysis
and radioactive isotopes.
X-ray
spectrometry: generation and properties of X-rays, instrumentation; electron spectroscopy
(Auger, ESCA). Atomic emission spectroscopy, properties of spectroscopic
techniques (flame, ICP optical emission spectrometry, atomic fluorescence
spectrometry), interferences, sensitivity, limits of detection, and analytical applications.
Atomic absorption spectroscopy: flame and electrothermal atomization, sources
of errors and background correction.
Separation
methods in analytical chemistry: Sample preparation (extractions, SPE). Gas chromatography (GC), high performance
liquid chromatography (HPLC), ion chromatography. Mass spectrometry:
ionization, analysis and detection of ions, identification of compounds.
Hyphenated
separation techniques gas (liquid) chromatography - mass spectrometry, elemental
mass spectrometry (ICP-MS)
Recommended reading: D.A. Skoog, F. J. Holler, S.R.
Crouch, Principles of Instrumental
Analysis, 6th Ed., Thomson Brooks Cole,
Analytical Chemistry A Modern Approach to Analytical Science, Ed. by
R. J.- Mermet, M. Otto, M. Valcarcel, Wiley - VCH, Weinheim, 2004;
J.C. Miller and J.N. Miller, Statistics
and Chemometrics for Analytical Chemistry, 4rd Ed., Prentice
Hall,
Teaching methods: Lectures
(45 hours) and seminars (30 hours) per week.
Assessment methods: Written asignement and oral examination
Language
of instruction: Slovenian
Last revision: 08/09/2011