Subject: SELECTED METHODS OF
INSTRUMENTAL ANALYSIS
Semester taught: 1st and 2nd
Programme of Study: Graduate study of chemistry
Pre-requisites: B. Sc. in Chemistry or Chemical Engineering
Objectives: On completion of this module the postgraduate student
will be able to: (I) to understand the principles and analytical performances
(capabilities) of modern instrumental methods; (II) to select a particular
method(s) for solving complex analytical problems.
Syllabus:
a) Spectrochemical analysis
Theoretical basis of optical spectroscopy;
Instrumentation in spectrochemical analysis; Flame, plasma, arc and spark
emission spectrometry; Atomic absorption spectrometry; Primary and secondary
X-ray spectrometry; Total reflection X-ray spectrometry.
Methodology and applications of spectrometric methods
in analytical chemistry; Flow injection spectrochemical analysis; the
application of spectroanalytical techniques in medicine, biology, environmental
sciences and industry.
b) Methods of ion and electron spectroscopy:
Methods of surface analysis and applications: X-ray
photoelectron spectroscopy (XPS); UV-photoelectron spectroscopy (UPS); Auger
electron spectroscopy (AES); Secondary ion mass spectrometry (SIMS); Laser
microprobe mass spectrometry (LMMS); Scanning electron and scanning tunneling
microprobes (SEM, STM); (II) Atomic mass spectrometry and applications:
Instrumentation (low vs. high
resolution instruments); isotopic analysis and applications in chemistry,
agriculture, geology (archeology), medicine; Multielemental analysis (ICP-MS,
SSMS); Isotope dilution mass spectrometry (IDMS); (III) Molecular mass
spectrometry and applications : Basic instrumentation; Ionization
techniques; Coupling of separation methods with mass spectrometry (GC-HPLC-MS);
Identification and structure elucidation of organic compounds; Trace analysis;
Process control.
c) Electroanalytical techniques
Potentiometry (sensors, ion selective electrodes,
application in studies of equilibria), precision techniques (controlled potential
electrolysis, coulometry).
Voltammetric techniques: pulse and differential pulse
polarography, square wave voltammetry, application of voltammetric techniques
in the determination of organic and inorganic species and studies of interactions
in solutions.
Stripping
techniques: anodic and cathodic stripping voltammetry, adsorption voltammetry,
application in trace analysis.
Trends and development of electroanalytical methods
and instrumentation: microelectrodes, chemically modified electrodes,
electrochemical detection in flow-through systems, hyphenated techniques.
Form of teaching: Lectures and seminars
Form of assessment: Examination
Recommended textbook:
D.A.
Skoog, F. J. Holler, S.R. Crouch, Principles of Instrumental
Analysis, 6th
Ed., Thompson Brooks/Cole, Belmont, CA, 2007.
White-Wood: Mass
Spectrometry-Applications in Science and Engineering, Wiley 1986.
J.D. Ingle Jr.,
S.R. Crouch, Spectrochemical analysis, Prentice Hall, Englewood Cliffs , New
Jersey 07632.
B. W. Rossiter, j. E Hamilton (Ed), Physical Methods of
Chemistry, Sec. Ed. Vol II, Electrochemical Methods, New York, 1986.
J. Wang, Stripping
analyiss, Principles, Instrumentation and Application, VCH Weinheim, 1985.